A nucleoside triphosphate for site-specific labelling of DNA by the Staudinger ligation.
نویسندگان
چکیده
A novel nucleotide building block for enzymatic synthesis of azide modified DNA and subsequent conjugation via Staudinger ligation was developed.
منابع مشابه
Synthesis of coumarin or ferrocene labeled nucleosides via Staudinger ligation
BACKGROUND Reaction of azides with triaryl phosphines under mild conditions gives iminophosphoranes which can react with almost any kind of electrophilic reagent, e.g. aldehydes/ketones to form imines or esters to form amides. This so-called Staudinger ligation has been employed in a wide range of applications as a general tool for bioconjugation including specific labeling of nucleic acids. ...
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ورودعنوان ژورنال:
- Chemical communications
دوره 18 شماره
صفحات -
تاریخ انتشار 2007